ISOLATION OF PROBIOTIC ENDOPHYTIC BACTERIA FROM A MEDICINAL PLANT AND IN-DEPTH CHARACTERIZATION OF BACILLUS ENDOPHYTICUS.

الملخص

This scientific article aims to isolate microorganisms with probiotic properties from the rhizosphere and endophytic tissues of medicinal plants, to characterize them in detail based on morphological, biochemical, and physiological attributes, and to conduct an in-depth analysis of specific features inherent to the Bacillus endophyticus strain. Modern standard microbiological protocols were applied, including sterile collection of plant samples, surface sterilization, tissue homogenization, and bacterial isolation on selective growth media.

The isolated microorganisms were analyzed through morphological examination (colony and cell structure), Gram staining, sporogenesis, catalase and oxidase reactions. Their fermentative activity was assessed using API test systems. Probiotic properties were evaluated based on acid tolerance at pH 2.0–3.0, resistance to 0.3–0.5% bile salts, auto-aggregation coefficient, and antagonistic activity against pathogens.

The results demonstrated the presence of probiotic-potential representatives such as Bacillus, Lactobacillus, and Enterococcus in medicinal plants. Notably, the Bacillus endophyticus strain exhibited superior metabolic activity, ecological adaptability, strong acid and bile tolerance, and effective inhibition zones against pathogenic microorganisms. This study confirms the potential of medicinal plant microbiota as a rich source of probiotics and substantiates the prospects of Bacillus endophyticus in biopharmaceutical and biotechnological applications.